Diarrhoeal enterotoxin production by strains of Bacillus thuringiensis isolated from commercial Bacillus thuringiensis-based insecticides
نویسندگان
چکیده
منابع مشابه
Human cell exposure assays of Bacillus thuringiensis commercial insecticides: production of Bacillus cereus-like cytolytic effects from outgrowth of spores.
Most contemporary bioinsecticides are derived from scaled-up cultures of Bacillus thuringiensis subspecies israelensis (Bti) and kurstaki (Btk), whose particulate fractions contain mostly B. thuringiensis spores (> 10(12)/L) and proteinaceous aggregates, including crystal-like parasporal inclusion bodies (PIB). Based on concerns over relatedness to B. cereus-group pathogens, we conducted extens...
متن کاملOccurrence of natural Bacillus thuringiensis contaminants and residues of Bacillus thuringiensis-based insecticides on fresh fruits and vegetables.
A total of 128 Bacillus cereus-like strains isolated from fresh fruits and vegetables for sale in retail shops in Denmark were characterized. Of these strains, 39% (50/128) were classified as Bacillus thuringiensis on the basis of their content of cry genes determined by PCR or crystal proteins visualized by microscopy. Random amplified polymorphic DNA analysis and plasmid profiling indicated t...
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Practically all Bacillus thuringiensis strains contain a set of self-replicating, extrachromosomal DNA molecules or plasmids, which vary in number and size in the different strains. The plasmid patterns obtained from gel electrophoresis have previously been used as a tool to characterize strains, but comparison of the plasmid patterns has been limited in the number and diversity of strains anal...
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The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28oC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40oC and pH 4.0, resp...
متن کاملPromise of Bacillus thuringiensis
Bacillus thuringiensis crystal (Cry) proteins are the most widely used biological insecticides in the world. Their value in safely controlling insects that destroy crops and transmit human diseases is well established.Here, we reviewB. thuringiensisCry proteins as novel anthelmintics with a unique mode of action. In laboratory studies, Cry proteins are highly effective against a broad range of ...
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ژورنال
عنوان ژورنال: FEMS Immunology and Medical Microbiology
سال: 1995
ISSN: 0928-8244
DOI: 10.1016/0928-8244(95)00079-4